mouse monoclonal anti hif1α (Novus Biologicals)
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Mouse Monoclonal Anti Hif1α, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+hif1%CE%B1/HIF-1+alpha+Antibody+(ESEE122)/pm40081516-105-10-13
Average 93 stars, based on 63 article reviews
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Blocking Assay:Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles. Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and Incubation:Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles. Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and |

