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mouse monoclonal anti hif1α  (Novus Biologicals)


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    Structured Review

    Novus Biologicals mouse monoclonal anti hif1α
    Mouse Monoclonal Anti Hif1α, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+hif1%CE%B1/HIF-1+alpha+Antibody+(ESEE122)/pm40081516-105-10-13
    Average 93 stars, based on 63 article reviews
    mouse monoclonal anti hif1α - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles.
    Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and mouse anti-HIF1α (Novus Biologicals, NB100-105; 1:50). ..

    Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles
    Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and mouse anti-HIF1α (Novus Biologicals, NB100-105; 1:50). ..

    Incubation:

    Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles.
    Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and mouse anti-HIF1α (Novus Biologicals, NB100-105; 1:50). ..

    Article Title: Saturation genome editing maps the functional spectrum of pathogenic VHL alleles
    Article Snippet: Cell permeabilization was carried out using 0.2% Triton X-100 (Thermo Fisher Scientific) for 10 min, followed by three washes with 1× PBST (1× DPBS and 0.1% Tween-20 (Thermo Fisher Scientific)). .. Samples were blocked by washing once and incubating in a blocking buffer (1× DPBS and 1% BSA (Sigma-Aldrich)) for 1 h. Samples were then incubated for 1 h at room temperature with primary antibodies in blocking buffer, including rabbit anti-VHL (Cell Signaling Technology, 68547; 1:200) and mouse anti-HIF1α (Novus Biologicals, NB100-105; 1:50). ..



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    OriGene hif1α
    Detection of FOXO3a ( a ), SOD2 ( b ), <t>HIF1α</t> ( c ), GLUT1 ( d ), and Bry ( e ) expression by immunohistochemistry. Immunohistochemical analysis was performed on IVD tissues with different Pfirrmann grades of degeneration. Protein levels were quantified by densitometric analysis of immunostaining using ImageJ software and expressed as the percentage of positive cells per area (five sections per sample; Pfirrmann I–II group, n = 6; Pfirrmann III group, n = 40 and Pfirrmann IV–V group, n = 9). Results are reported as a whisker box plot representing the min to max (the line indicates median). * p < 0.01 (Pfirrmann III group vs. Pfirrmann I–II group); ^ p < 0.01 (Pfirrmann IV–V group vs. Pfirrmann I–II group); § p < 0.01 (Pfirrmann III group vs. Pfirrmann IV–V group). Scale bars 20 μm
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    R&D Systems anti hif1α
    Detection of FOXO3a ( a ), SOD2 ( b ), <t>HIF1α</t> ( c ), GLUT1 ( d ), and Bry ( e ) expression by immunohistochemistry. Immunohistochemical analysis was performed on IVD tissues with different Pfirrmann grades of degeneration. Protein levels were quantified by densitometric analysis of immunostaining using ImageJ software and expressed as the percentage of positive cells per area (five sections per sample; Pfirrmann I–II group, n = 6; Pfirrmann III group, n = 40 and Pfirrmann IV–V group, n = 9). Results are reported as a whisker box plot representing the min to max (the line indicates median). * p < 0.01 (Pfirrmann III group vs. Pfirrmann I–II group); ^ p < 0.01 (Pfirrmann IV–V group vs. Pfirrmann I–II group); § p < 0.01 (Pfirrmann III group vs. Pfirrmann IV–V group). Scale bars 20 μm
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    Novus Biologicals mouse monoclonal anti hif1α
    Detection of FOXO3a ( a ), SOD2 ( b ), <t>HIF1α</t> ( c ), GLUT1 ( d ), and Bry ( e ) expression by immunohistochemistry. Immunohistochemical analysis was performed on IVD tissues with different Pfirrmann grades of degeneration. Protein levels were quantified by densitometric analysis of immunostaining using ImageJ software and expressed as the percentage of positive cells per area (five sections per sample; Pfirrmann I–II group, n = 6; Pfirrmann III group, n = 40 and Pfirrmann IV–V group, n = 9). Results are reported as a whisker box plot representing the min to max (the line indicates median). * p < 0.01 (Pfirrmann III group vs. Pfirrmann I–II group); ^ p < 0.01 (Pfirrmann IV–V group vs. Pfirrmann I–II group); § p < 0.01 (Pfirrmann III group vs. Pfirrmann IV–V group). Scale bars 20 μm
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    GeneTex mouse anti- hif1α antibody gtx640664
    The conditioned medium (CM) of Sirt1 -Tg CSMCs inhibits the proliferation of Caco-2 cells in vitro. ( A ) qRT-PCR of cZFP609 expression in CSMCs from WT or Sirt1 -Tg mice treated with TNF-α for 24 h. ( B ) qRT-PCR of cZFP609 expression in Caco-2 cells incubated with the TNF-α-induced CSMC conditioned medium (CM) for 24 h and exposed to hypoxia. ( C ) Ratio of cell counting of Caco-2 cells incubated with the TNF-α-induced CSMC (CM) for 24 h and exposed to hypoxia. ( D , E ) Migration of Caco-2 cells was assessed using a scratch wound assay. Caco-2 cells were incubated with the TNF-α-induced CSMC exosomes (CM) for 24 h and exposed to hypoxia treated with TNF-α. (Scale bar: 200 µm.) Data are presented as mean ± SEM. ( F ) Immunofluorescent confocal microscopy of <t>HIF1α</t> nuclear translocation in the Caco-2 cells, Scale bars: 25 μm. Bar graphs show mean ± SEM. Student’s t -test or one-way ANOVA was used. ** p < 0.01 versus the corresponding control.
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    GeneTex mouse anti- hif1α antibody
    The conditioned medium (CM) of Sirt1 -Tg CSMCs inhibits the proliferation of Caco-2 cells in vitro. ( A ) qRT-PCR of cZFP609 expression in CSMCs from WT or Sirt1 -Tg mice treated with TNF-α for 24 h. ( B ) qRT-PCR of cZFP609 expression in Caco-2 cells incubated with the TNF-α-induced CSMC conditioned medium (CM) for 24 h and exposed to hypoxia. ( C ) Ratio of cell counting of Caco-2 cells incubated with the TNF-α-induced CSMC (CM) for 24 h and exposed to hypoxia. ( D , E ) Migration of Caco-2 cells was assessed using a scratch wound assay. Caco-2 cells were incubated with the TNF-α-induced CSMC exosomes (CM) for 24 h and exposed to hypoxia treated with TNF-α. (Scale bar: 200 µm.) Data are presented as mean ± SEM. ( F ) Immunofluorescent confocal microscopy of <t>HIF1α</t> nuclear translocation in the Caco-2 cells, Scale bars: 25 μm. Bar graphs show mean ± SEM. Student’s t -test or one-way ANOVA was used. ** p < 0.01 versus the corresponding control.
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    R&D Systems hif1α
    The conditioned medium (CM) of Sirt1 -Tg CSMCs inhibits the proliferation of Caco-2 cells in vitro. ( A ) qRT-PCR of cZFP609 expression in CSMCs from WT or Sirt1 -Tg mice treated with TNF-α for 24 h. ( B ) qRT-PCR of cZFP609 expression in Caco-2 cells incubated with the TNF-α-induced CSMC conditioned medium (CM) for 24 h and exposed to hypoxia. ( C ) Ratio of cell counting of Caco-2 cells incubated with the TNF-α-induced CSMC (CM) for 24 h and exposed to hypoxia. ( D , E ) Migration of Caco-2 cells was assessed using a scratch wound assay. Caco-2 cells were incubated with the TNF-α-induced CSMC exosomes (CM) for 24 h and exposed to hypoxia treated with TNF-α. (Scale bar: 200 µm.) Data are presented as mean ± SEM. ( F ) Immunofluorescent confocal microscopy of <t>HIF1α</t> nuclear translocation in the Caco-2 cells, Scale bars: 25 μm. Bar graphs show mean ± SEM. Student’s t -test or one-way ANOVA was used. ** p < 0.01 versus the corresponding control.
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    Image Search Results


    Detection of FOXO3a ( a ), SOD2 ( b ), HIF1α ( c ), GLUT1 ( d ), and Bry ( e ) expression by immunohistochemistry. Immunohistochemical analysis was performed on IVD tissues with different Pfirrmann grades of degeneration. Protein levels were quantified by densitometric analysis of immunostaining using ImageJ software and expressed as the percentage of positive cells per area (five sections per sample; Pfirrmann I–II group, n = 6; Pfirrmann III group, n = 40 and Pfirrmann IV–V group, n = 9). Results are reported as a whisker box plot representing the min to max (the line indicates median). * p < 0.01 (Pfirrmann III group vs. Pfirrmann I–II group); ^ p < 0.01 (Pfirrmann IV–V group vs. Pfirrmann I–II group); § p < 0.01 (Pfirrmann III group vs. Pfirrmann IV–V group). Scale bars 20 μm

    Journal: Histochemistry and Cell Biology

    Article Title: Immunohistochemical analysis to detect a molecular signature in intervertebral disc degeneration

    doi: 10.1007/s00418-025-02434-w

    Figure Lengend Snippet: Detection of FOXO3a ( a ), SOD2 ( b ), HIF1α ( c ), GLUT1 ( d ), and Bry ( e ) expression by immunohistochemistry. Immunohistochemical analysis was performed on IVD tissues with different Pfirrmann grades of degeneration. Protein levels were quantified by densitometric analysis of immunostaining using ImageJ software and expressed as the percentage of positive cells per area (five sections per sample; Pfirrmann I–II group, n = 6; Pfirrmann III group, n = 40 and Pfirrmann IV–V group, n = 9). Results are reported as a whisker box plot representing the min to max (the line indicates median). * p < 0.01 (Pfirrmann III group vs. Pfirrmann I–II group); ^ p < 0.01 (Pfirrmann IV–V group vs. Pfirrmann I–II group); § p < 0.01 (Pfirrmann III group vs. Pfirrmann IV–V group). Scale bars 20 μm

    Article Snippet: For immunohistochemical evaluation, sections were incubated overnight (4 °C) with a primary antibody against FOXO3a (#ab70315, rabbit anti-human, 1:100 dilution; Abcam, Cambridge, UK), SOD2 (#sc-133134, mouse anti-human, 1:100 dilution; Santa Cruz biotech., Dallas, USA), HIF1α (clone H1alpha67, mouse anti-human, 1:200 dilution; Novusbio, Centennial, USA), GLUT1 (#TA301678, mouse anti-human, 1:200 dilution; Origene, Rockville, USA), and BRY (#ab209665, rabbit anti-human, 1:500 dilution; Abcam), followed by treatment with Vectastain ABC solution (#MP-7500; Vectorlabs, Burlingame, USA) for 30 min. To achieve specificity, the working concentrations of primary antibodies were selected by following the dilution guidelines and technical specifications provided by the antibody’s manufacturer.

    Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Immunostaining, Software, Whisker Assay

    Analysis of FOXO3a, SOD2, HIF1α, GLUT1, and Bry expression in IVD tissues with Pfirrmann grade III. Protein levels were assessed by immunohistochemistry and quantified by densitometric analysis of immunostaining using ImageJ software. Results were expressed as the percentage of positive cells per area, based on five representative sections per sample ( n = 40, Pfirrmann grade III). Data are presented as box-and-whisker plots, displaying min to max (the line indicates the median). Comparisons were made on the basis of the following parameters: a sex (female, male), age (18–40, 41–60, > 60 years), smoking status (non-smoker [NS], former smoker [FS], current smoker [CS]), and body mass index (BMI) (normal weight [NW], overweight [OW], obese [OB]); b anatomical site of surgery (L2–L3, L3–L4, L4–L5, L5–S1), duration of symptoms before surgery (< 6 months, ≥ 6 months), and area of infiltration (moderate, abundant)

    Journal: Histochemistry and Cell Biology

    Article Title: Immunohistochemical analysis to detect a molecular signature in intervertebral disc degeneration

    doi: 10.1007/s00418-025-02434-w

    Figure Lengend Snippet: Analysis of FOXO3a, SOD2, HIF1α, GLUT1, and Bry expression in IVD tissues with Pfirrmann grade III. Protein levels were assessed by immunohistochemistry and quantified by densitometric analysis of immunostaining using ImageJ software. Results were expressed as the percentage of positive cells per area, based on five representative sections per sample ( n = 40, Pfirrmann grade III). Data are presented as box-and-whisker plots, displaying min to max (the line indicates the median). Comparisons were made on the basis of the following parameters: a sex (female, male), age (18–40, 41–60, > 60 years), smoking status (non-smoker [NS], former smoker [FS], current smoker [CS]), and body mass index (BMI) (normal weight [NW], overweight [OW], obese [OB]); b anatomical site of surgery (L2–L3, L3–L4, L4–L5, L5–S1), duration of symptoms before surgery (< 6 months, ≥ 6 months), and area of infiltration (moderate, abundant)

    Article Snippet: For immunohistochemical evaluation, sections were incubated overnight (4 °C) with a primary antibody against FOXO3a (#ab70315, rabbit anti-human, 1:100 dilution; Abcam, Cambridge, UK), SOD2 (#sc-133134, mouse anti-human, 1:100 dilution; Santa Cruz biotech., Dallas, USA), HIF1α (clone H1alpha67, mouse anti-human, 1:200 dilution; Novusbio, Centennial, USA), GLUT1 (#TA301678, mouse anti-human, 1:200 dilution; Origene, Rockville, USA), and BRY (#ab209665, rabbit anti-human, 1:500 dilution; Abcam), followed by treatment with Vectastain ABC solution (#MP-7500; Vectorlabs, Burlingame, USA) for 30 min. To achieve specificity, the working concentrations of primary antibodies were selected by following the dilution guidelines and technical specifications provided by the antibody’s manufacturer.

    Techniques: Expressing, Immunohistochemistry, Immunostaining, Software, Whisker Assay

    Correlation between FOXO3a, SOD2, HIF1α, GLUT1, and Bry expression levels and the onset of chronic pain or relapse. Results were expressed as percentage of positive cells per area, based on five representative sections per sample ( n = 24, Pfirrmann grade III). Data are presented as box-and-whisker plots, displaying min to max (the line indicates the median). Patients without chronic pain after surgery (green), with joint inflammatory symptoms after several months (blue), and with short-term relapses (red)

    Journal: Histochemistry and Cell Biology

    Article Title: Immunohistochemical analysis to detect a molecular signature in intervertebral disc degeneration

    doi: 10.1007/s00418-025-02434-w

    Figure Lengend Snippet: Correlation between FOXO3a, SOD2, HIF1α, GLUT1, and Bry expression levels and the onset of chronic pain or relapse. Results were expressed as percentage of positive cells per area, based on five representative sections per sample ( n = 24, Pfirrmann grade III). Data are presented as box-and-whisker plots, displaying min to max (the line indicates the median). Patients without chronic pain after surgery (green), with joint inflammatory symptoms after several months (blue), and with short-term relapses (red)

    Article Snippet: For immunohistochemical evaluation, sections were incubated overnight (4 °C) with a primary antibody against FOXO3a (#ab70315, rabbit anti-human, 1:100 dilution; Abcam, Cambridge, UK), SOD2 (#sc-133134, mouse anti-human, 1:100 dilution; Santa Cruz biotech., Dallas, USA), HIF1α (clone H1alpha67, mouse anti-human, 1:200 dilution; Novusbio, Centennial, USA), GLUT1 (#TA301678, mouse anti-human, 1:200 dilution; Origene, Rockville, USA), and BRY (#ab209665, rabbit anti-human, 1:500 dilution; Abcam), followed by treatment with Vectastain ABC solution (#MP-7500; Vectorlabs, Burlingame, USA) for 30 min. To achieve specificity, the working concentrations of primary antibodies were selected by following the dilution guidelines and technical specifications provided by the antibody’s manufacturer.

    Techniques: Expressing, Whisker Assay

    The conditioned medium (CM) of Sirt1 -Tg CSMCs inhibits the proliferation of Caco-2 cells in vitro. ( A ) qRT-PCR of cZFP609 expression in CSMCs from WT or Sirt1 -Tg mice treated with TNF-α for 24 h. ( B ) qRT-PCR of cZFP609 expression in Caco-2 cells incubated with the TNF-α-induced CSMC conditioned medium (CM) for 24 h and exposed to hypoxia. ( C ) Ratio of cell counting of Caco-2 cells incubated with the TNF-α-induced CSMC (CM) for 24 h and exposed to hypoxia. ( D , E ) Migration of Caco-2 cells was assessed using a scratch wound assay. Caco-2 cells were incubated with the TNF-α-induced CSMC exosomes (CM) for 24 h and exposed to hypoxia treated with TNF-α. (Scale bar: 200 µm.) Data are presented as mean ± SEM. ( F ) Immunofluorescent confocal microscopy of HIF1α nuclear translocation in the Caco-2 cells, Scale bars: 25 μm. Bar graphs show mean ± SEM. Student’s t -test or one-way ANOVA was used. ** p < 0.01 versus the corresponding control.

    Journal: International Journal of Molecular Sciences

    Article Title: Smooth Muscle Silent Information Regulator 1 Contributes to Colitis in Mice

    doi: 10.3390/ijms26051807

    Figure Lengend Snippet: The conditioned medium (CM) of Sirt1 -Tg CSMCs inhibits the proliferation of Caco-2 cells in vitro. ( A ) qRT-PCR of cZFP609 expression in CSMCs from WT or Sirt1 -Tg mice treated with TNF-α for 24 h. ( B ) qRT-PCR of cZFP609 expression in Caco-2 cells incubated with the TNF-α-induced CSMC conditioned medium (CM) for 24 h and exposed to hypoxia. ( C ) Ratio of cell counting of Caco-2 cells incubated with the TNF-α-induced CSMC (CM) for 24 h and exposed to hypoxia. ( D , E ) Migration of Caco-2 cells was assessed using a scratch wound assay. Caco-2 cells were incubated with the TNF-α-induced CSMC exosomes (CM) for 24 h and exposed to hypoxia treated with TNF-α. (Scale bar: 200 µm.) Data are presented as mean ± SEM. ( F ) Immunofluorescent confocal microscopy of HIF1α nuclear translocation in the Caco-2 cells, Scale bars: 25 μm. Bar graphs show mean ± SEM. Student’s t -test or one-way ANOVA was used. ** p < 0.01 versus the corresponding control.

    Article Snippet: The cells were then incubated with mouse anti- HIF1α (1:100, GTX640664, GeneTex, Irvine, CA, USA) antibody at 4 °C overnight.

    Techniques: In Vitro, Quantitative RT-PCR, Expressing, Incubation, Cell Counting, Migration, Scratch Wound Assay Assay, Confocal Microscopy, Translocation Assay, Control

    cZFP609 inhibits proliferation in Caco-2 cells via inhibiting HIF1α nuclear translocation. ( A ) qRT-PCR of cZFP609 expression in Caco-2 cells treated with vector or cZFP609. ( B ) Ratio of cell counting of Caco-2 cells treated with vector or cZFP609. ( C , D ) Migration of Caco-2 cells was assessed using a scratch wound assay. Caco-2 cells treated with vector of cZFP609. (Scale bar: 200 µm). Data are presented as mean ± SEM. ( E ) Immunofluorescent confocal microscopy of HIF1α nuclear translocation in the Caco-2 cells. (Scale bars: 25 μm). Bar graphs show mean ± SEM. Student’s t -test or one-way ANOVA was used. ** p < 0.01 versus the corresponding control.

    Journal: International Journal of Molecular Sciences

    Article Title: Smooth Muscle Silent Information Regulator 1 Contributes to Colitis in Mice

    doi: 10.3390/ijms26051807

    Figure Lengend Snippet: cZFP609 inhibits proliferation in Caco-2 cells via inhibiting HIF1α nuclear translocation. ( A ) qRT-PCR of cZFP609 expression in Caco-2 cells treated with vector or cZFP609. ( B ) Ratio of cell counting of Caco-2 cells treated with vector or cZFP609. ( C , D ) Migration of Caco-2 cells was assessed using a scratch wound assay. Caco-2 cells treated with vector of cZFP609. (Scale bar: 200 µm). Data are presented as mean ± SEM. ( E ) Immunofluorescent confocal microscopy of HIF1α nuclear translocation in the Caco-2 cells. (Scale bars: 25 μm). Bar graphs show mean ± SEM. Student’s t -test or one-way ANOVA was used. ** p < 0.01 versus the corresponding control.

    Article Snippet: The cells were then incubated with mouse anti- HIF1α (1:100, GTX640664, GeneTex, Irvine, CA, USA) antibody at 4 °C overnight.

    Techniques: Translocation Assay, Quantitative RT-PCR, Expressing, Plasmid Preparation, Cell Counting, Migration, Scratch Wound Assay Assay, Confocal Microscopy, Control